脂肪酸结合蛋白载脂蛋白E基因多态性与2型糖尿病并发冠心病的关系.docVIP

脂肪酸结合蛋白、载脂蛋白E基因多态性与2型糖尿病并发冠心病的关系 　　作者：石玉荣，周海艳，章 尧 作者单位：蚌埠医学院：1. 生化与分子生物学教研室;2. 临床检验诊断学实验中心;3. 第一附属医院老干科，安徽蚌埠 233000 　　【摘要】 目的 探讨2型糖尿病(T2DM)患者脂肪酸结合蛋白(FABP2)及载脂蛋白E(ApoE)与冠心病(CHD)的关系。方法 采用聚合酶链反应限制性片段长度多态性(PCRPFLP)技术分别检测T2DM并发CHD组及T2DM非CHD组FABP2、ApoE基因型，并检测T2DM并发CHD组不同等位基因携带者血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HLDC)、低密度脂蛋白胆固醇(LDLC)。结果 T2DM并发CHD组FABP2 A/T突变频率高于非冠心病组(P0.05)，T等位基因携带者与A等位基因携带者相比TG、TC、LDLC浓度升高，血HDLC浓度降低，有显著性差异(P0.05);T2DM并CHD组Ε4等位基因频率明显高于对照组(P0.01)，Ε4等位基因携带者与Ε3等位基因携带者相比TC、LDLC浓度升高，HDLC浓度降低，差异有统计学意义(P0.05)，FABP与ApoE基因连锁分析，FABP2T基因和ApoEΕ4基因连锁时，T2DM并发CHD的几率增加(P0.05)。结论 FABP2 T基因及ApoE/Ε4等位基因增高T2DM并发CHD的遗传易感性。 　　【关键词】 糖尿病;冠心病;脂肪酸结合蛋白;基因多态性;载脂蛋白E 　　and apolipoprotein E with coronary heart disease 　　in type2 diabetic patientsSHI Yurong1,2, ZHOU Haiyan3, ZHANG Yao1 　　(1. Department of Biochemistry and Molecular Biology; 2. Research Center of 　　Clinical Laboratory Science; 3. Department of Agedness of the First Affiliated Hospital, 　　Bengbu Medical College, Bengbu 233000, China)ABSTRACT: Objective To study the relationship of fatty acid binding protein2 (FABP2) and apolipoprotein E (ApoE) with coronary heart disease (CHD) in type2 diabetes mellitus (T2DM) patients. Methods FABP2 and ApoE gene polymorphisms were detected by PCRRFLP. At the same time all CHD in T2DM patients serum triglyceride (TG), total cholesterol (TC), highdensity lipoprotein cholesterol were (HDLC), lowdensity lipoprotein cholesterol (LDLC) were detected. Results The Tallele frequencies of FABP2 were higher (P0.05), the levels of serum TC, TG and LDLC of Tallele carriers were higher while HDLC was lower than that of Aallele carriers (P0.05). The Ε3allele frequencies of ApoE were higher (P0. 01), the levels of serum TC and LDLC of Tallele carriers were higher, but HDLC was lower than that of Ε3allele carriers (P0.05). Combined analysis of FABP2 and APOE genotype showed that the FABP2T+/APOEΕ4+genotype was a risk factor of CHD in T2DM patients (P0.05). C