WesternBlot图片灰度分析ImageJ.docVIP

Western Blot图片灰度分析 -Image JThis method is the Gel Analysis method outlined in the ImageJ documentation: Gel Analysis . You may prefer to use it instead of the methods outlined below. There will likely be very little difference in the results between the various methods Open your file. Go to AnalyzeGelsGel Analyzer Options and click the boxes for Label With Percentages, Outline Lanes and Invert Peaks. Choose the Rectangular Selection tool. Draw a rectangle around your first lane. Encompass some area of the lane above and below the band of interest. Edit July 2009: Note that for a gel with the lanes oriented vertically as shown here (i.e. the visible bands are horizontal across the image), you want to make your bounding rectangle taller than it is wide. However, if you have the image rotated so that the lanes are running horizontally, you need to make your bounding rectangle at least twice as wide as it is tall, at which point Image-J will recognize that your lanes are horizontal and it will allow you to move the box up or down the image to enclose the neighboring lanes. Press the 1 button (or go to AnalyzeGelsSelect First Lane). A new window will pop up with a copy of your image and a label over your first rectangular selection. Use the arrow keys to move the rectangle over the next lane. Press 2 (or go to AnalyzeGelsSelect Next Lane) to place a selection around the lane. Repeat this for each lane on the membrane, moving the box and pressing 2 to place the selection. When finished, press 3 (or go to AnalyzeGelsPlot Lanes), which pops up a new window with a profile plot of each lane. Now choose the Straight Line selection tool. At the base of each peak, draw a line from one side of the peak to the other. This encloses the area of the peak. The tails to either side of the peak are the background signal. Note that if you have many lanes, the later lanes will be hidden at the bottom of the profile plot. To see these lanes, press and hold the space bar, and use the mouse