外文资料Transcriptional dynamics of the developing sweet cherry (Prunus avium L.) fruit sequencing, annotation and expression profiling of exocarp-associated genes.pdfVIP

OPEN Citation: Horticulture Research (2014) 1, 11; doi:10.1038/hortres.2014.11 2014 Nanjing Agricultural University All rights reserved 2052-7276/14 /hortres ARTICLE Transcriptional dynamics of the developing sweet cherry (Prunus avium L.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes 1 1 1 2 1 Merianne Alkio , Uwe Jonas , Myriam Declercq , Steven Van Nocker and Moritz Knoche The exocarp, or skin, of fleshy fruit is a specialized tissue that protects the fruit, attracts seed dispersing fruit eaters, and has large economical relevance for fruit quality. Development of the exocarp involves regulated activities of many genes. This research analyzed global gene expression in the exocarp of developing sweet cherry (Prunus avium L., ‘Regina’), a fruit crop species with little public genomic resources. A catalog of transcript models (contigs) representing expressed genes was constructed from de novo assembled short complementary DNA (cDNA) sequences generated from developing fruit between flowering and maturity at 14 time points. Expression levels in each sample were estimated for 34 695 contigs from numbers of reads mapping to each contig. Contigs were annotated functionally based on BLAST, gene ontology and InterProScan analyses. Coregulated genes were detected using partitional clustering of expression patterns. The results are discussed with emphasis on genes putatively involved in cuticle deposition, cell wall metabolism and sugar transport. The high temporal resolution of the expression patterns presented here reve