外文资料Molecular Characterization of Berberine Bridge Enzyme Genes from Opium oppy.pdfVIP

Plant Physiol. (1996) 112: 1669-1 677 Molecular Characterization of Berberine Bridge Enzyme Genes from Opium ~oppy Peter J. Facchini*, Catherine Penzes, Alison C. Johnson, and Dean Bull Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada T2N 1N4 addition of various elicitors has been demonstrated for In Papaver somniferum (opium poppy) and related species, (S)- cell-suspension cultures of Eschscholtzia californica, the Cal- reticuline serves as a branch-point intermediate in the biosynthesis ifornia poppy (Schumacher et al., 1987), and Papaver som- of numerous isoquinoline alkaloids. The berberine bridge enzyme niferum, the opium poppy (Eilert et al., 1985). (BBE) ([SI-reticu1ine:oxygen oxidoreductase [methylene bridge (S)-Reticuline also serves as the universal biosynthetic forming], EC ) catalyzes the stereospecific conversion of the precursor to a variety of other isoquinoline alkaloids in N-methyl moiety of (9-reticuline into the berberine bridge carbon plants. In some members of the genus Papaver, (S)- of (9-scoulerine and represents the first committed step in the reticuline is converted to its (X)-epimer to initiate the bio- pathway leading to the antimicrobial alkaloid sanguinarine. Three unique genomic clones (bbel, bbe2, and bbe3) similar to a BBE synthetic pathway to morphine (Weiczorek et al., 1986) cDNA from fschscholtzia californica (California poppy) were iso- (Fig. 1).In aerial organs of opium poppy, morphine is the