Progress of liquid chromatography-mass spectrometry in measurement of vitamin D metabolites and analogue.pdf

Clinical Biochemistry 44 (2011) 66–76 Contents lists available at ScienceDirect Clinical Biochemistry journal homepage: www.else vi /l oca te/clinbiochem Review Progress of liquid chromatography-mass spectrometry in measurement of vitamin D metabolites and analogues Joe M. El-Khoury a,b, Edmunds Z. Reineks a, Sihe Wang a,b,⁎ a Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA b Department of Chemistry, Cleveland State University, Cleveland, OH, USA a r t i c l e i n f o a b s t r a c t Article history: Objective: Clinical testing for vitamin D nutritional status has experienced tremendous growth in the past Received 16 March 2010 several years, driven by research results linking various diseases with low serum 25-hydroxyvitamin D [25 Received in revised form 5 May 2010 (OH)D] levels. Meanwhile, interest in the pathophysiological mechanism elucidation and pharmaceutical Accepted 10 May 2010 applications requires measurement of vitamin D metabolites and analogues. Liquid chromatography-mass Available online 21 May 2010 spectrometry (LC-MS) has been increasingly utilized in these applications. In this work, our objective was to critically review the progress of LC-MS application in measuring vitamin D metabolites and analogues in Keywords: Vitamin D biological fluids. 25-hydroxyvitamin D