生物蛋白纯化论文.docVIP

Cement protein的转化表达优化与纯化 摘要:本实验将含有Cement protein基因片段的重组载体pCold TF转化入E.coli BL21(DE3)中, 在摇瓶培养的条件下，细胞中融合蛋白的含量达到60%以上；然后利用包涵体处理、复性、Ni-IDA亲和层析的方法纯化蛋白。使用2.4L的菌液，经诱导、纯化、复性后大约可以得到10mg的蛋白；同时采用ELISA检测法，证实了纯化的SPG有很高的活性。 关键词：融合蛋白的表达；Ni-IDA亲和层析。 Abstract: In this study, containing Cement protein fragment pCold TF recombinant vector was transformed into E.coli BL21 (DE3), in shake flask culture conditions, cell protein content above 60%; Then inclusion treatment, renaturation, Ni-IDA affinity chromatography purified protein. Use 2.4L of bacteria, by induction, purification, can be refolded about 10mg of p