PEG渗透胁迫下玉米幼苗叶片保护酶活性昼夜变化.pdf

安徽农业科学，JounudofAnhui^删．Sei．2011。39(12)：6959—6962 责任编辑张彩丽责任校对傅真治 PEG渗透胁迫下玉米幼苗叶片保护酶活性昼夜变化 杨玲，郭敏 (东北林业大学林学院，林木遗传育种与生物技术教育部重点实验室，黑龙江哈尔滨150040) 果]一昼夜胁迫中，保护酶活性的变化不仅受PEG浓度和胁迫时间的影响，而且受先照的影响。随着光照时间和胁迫时闻的延长，叶片 电解质外渗率和MDA含量增加、SOD活性持续降低、POD和CAT活性的变化率则呈先增加后降低趋势；光照结束后，随着黑暗时间的 延长，电解质外渗率和保护酶活性先接近对照水平，然后发生较大渡动。[结论】玉米幼苗叶片的保护酶活性变化不仅受PEG浓度、胁 迫时间的影响，而且受光照的影响。 关键词玉米幼苗；保护酶海|生；昼夜变化；PEG胁迫 “ 中图分类号Q945．78文献标识码A 文章编号0517—6611(2011)12—06959—04 DiurnalVariationofoeⅡDefense inLeavesofMaize under Stress EnzymeActivity SeedlingPolyethyleneGlycol一60()o YANGetal ofEducation ofForestTreeGenetic and ofForestry， Lmg (Ministry KeyLaboratory ImprovementBiotechnology，School Northeast 150040) ForestryUniversity，Harbin．Heilongjiang Abstract researchaimedto theinfluenceofstreg$onthediurnalvariationofcelldefense in Objective)The study enzymeactivityplants． MethodEffectsofosmoticstressonthediurnalvariationof and inactivitiesofthemainantioxidant I electrolyteleakagechange enzymes，in- leavesofmaize were withdifferent ehudingsuperoxidedismutase(SOD)，peroxidase(POD)andcatalase(CAT)，inseedlingsinvestigated concentrationsof resultsshowedthe of not be Result1 11Ie that activity enzymesonly polyethyleneglycol(PEG-6000)treatment．1 protective the andstress also tIIe time stresstime by PEGconcentrationtime。but hghtinoneround山eclock．AsiRuminationand impacted by prolong，