常低压低氧高二氧化碳动物实验舱的实验研究.pdfVIP

Chinese Journal of Medical Instrumentation 2010 年34 卷第2 期 研 究 与 论 著 文章编号 1671-7104(2010)02-0102-03 ： 常低压低氧高二氧化碳动物实验舱的实验研究 【作 者】种银保* ，刘九零，赵安，王晴 第三军医大学新桥医院设备科，重庆市，400037 【摘 要】 目的 探讨常低压低氧高二氧化碳实验动物舱复制动物模型的可行性。方法 选择雄性SPF级SD大鼠60只进行分组研究，20 只做常压低氧组对照实验，40只做低压低氧组对照实验。对每组研究利用多功能生理检测仪测量大鼠的肺动脉压和颈动脉 压指标，并观察肺血管结构的变化。结果 常低压低氧高二氧化碳环境可以促进肺动脉高压的形成，加速肺血管重构改变， 促右室肥厚。结论 临床应用表明，该舱测控准确、安全可靠、重复性好；能成功复制常低压低氧高二氧化碳环境下的肺动 脉高压模型，为研究各种因缺氧而导致的各种循环与呼吸系统疾病的生理机理提供了一种实验技术平台。 【关 键 词】常压；低压；低氧；高二氧化碳；动物实验舱；肺动脉高压模型 【中图分类号】R318 【文献标识码】 A Experimental Study of Animal Cabin with ATM/LP and Low Oxygen and High Carbon Dioxide 【Writers 】Chong Yinbao, Liu Jiuling, Zhao An, Wang Qing Equipment Division of Xinqiao Hospital, Third Military Medical University, Chongqing, 400037 【Abstract 】Objective To explore the feasibility of the animal experimental cabin to copy the animal models with ATM/LP and low oxygen and high carbon dioxide environment. Methods 60 SPF-class male SD rats were divided into two groups, 20 for normobaric hypoxia control experiments and the other 40 for hypobaric hypoxia control experiments. For each group, examine the pulmonary arterial pressure and carotid arterial pressure indicators of rats by using the multi-detector measurement of physiological, and observe the pulmonary vascular changes in the structure. Results The ATM/LP and low oxygen and high carbon dioxide environment can promote the formation of pulmonary hypertension and accelerate changes in pulmonary vascular remodeling, promoting right ventricular hypertrophy. Conclusion Clinical applications showed that the animal experimental cabin was observing and controlling accurately, safe, reliable, and the results was reproducible. It can su