抑制Dicer基因对shRNA功能发挥地影响.pdfVIP

第 21 卷 6 期 中 国 病 毒 学 21 （6 ）：604-608 2006 年 11 月 VIROLOGICA SINICA November 2006 * 抑制 Dicer 基因对 shRNA 功能发挥的影响 张秉强 1 ， 陈维贤 1 ，黄 英 1 ，何茂锐 1 ， 吴 莹 1 ， 张 君 1 ， Tong-Chuan He2 ， 黄爱龙 1 ** (1. 重庆医科大学省部共建感染性疾病分子生物学教育部重点实验室 重庆医科大学病毒性肝炎研究所，重庆，400016; 2. Molecular Oncology Laboratory, The University of Chicago Medical Center, IL 60637, USA) Inhibition of Dicer Gene and Its Influence on the Function of shRNA 1 1 1 1 1 1 ZHANG Bing-qiang , CHEN Wei-xian , HUANG Ying , HE Mao-rui , WU Ying , ZHANG Jun , 2 1** TONG-Chuang HE , HUANG Ai-long (1.The Key Laboratory of Molecular Biology of Infectious Diseases of Ministry of Education, The Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400016, China；2 ．Molecular Oncology Laboratory, The University of Chicago Medical Center, IL 60637,USA) Abstract: In order to determine the effect of silencing Dicer on shRNA-mediated RNA interference, we constructed two shRNA expression vectors that targeted the RNAse III domain of the Dicer gene. The shRNA vectors were transfected into 2215 cells, colon cancer TC cells and Green Fluorescent Protein (GFP)-tagged HepG2 A9 cells using lipofectamineTM 2000. The level of Dicer mRNA was assessed by RT-PCR. After the knocking-down