损伤三叉神经SAMP8小鼠学习记忆相关脑区胆碱能神经元减少及学习能力下降：存在年龄差异.docVIP

损伤三叉神经SAMP8小鼠学习记忆相关脑区胆碱能神经元减少及学习能力下降：存在年龄差异 牙齿与中枢神经有直接联系的是三叉神经感觉传导通路，那么牙齿损害引起的中枢神经损害是否与三叉神经损害有关？为解决这一问题，中国中山大学光华口腔医学院的何依帆博士所在课题组选用快速老化痴呆模型SAMP8小鼠，分别于4（中年）和7月龄（老年）时横断三叉神经传导通路中重要的外周神经--下牙槽神经，以相应鼠龄行假手术小鼠为对照组。结果发现，与老年对照组小鼠比较，切断下牙槽神经的老年大鼠海马CA1区、CA3区锥体细胞层神经元数量明显减少，海马CA1、齿状回乙酰胆碱酯酶AchE）阳性（胆碱能）神经纤维均明显减少，膈内侧核和斜角带垂直支胆碱乙酰转移酶（ChAT）免疫阳性（胆碱能）神经元数量明显减少，学习能力明显下降。但切断下牙槽神经对中年小鼠学习记忆相关脑区胆碱能神经元无明显影响。研究结果提示，三叉神经损害可引起隔-海马胆碱能投射通路退变和海马锥体细胞神经元的丢失，进而引起学习能力降低，三叉神经损害对学习记忆相关脑区胆碱能神经元和学习能力的影响存在年龄差异，故若老年人出现三叉神经损伤时应慎用拔牙和神经损害性治疗，以延缓相关脑区胆碱能神经元减少所致的神经退行性疾病的发生。相关内容发表于《中国神经再生研究（英文版）》杂志2014年11月第22期。 Article: Age-dependent loss of cholinergic neurons in learning and memory-related brain regions and impaired learning in SAMP8 mice with trigeminal nerve damage by Yifan He1, Jihong Zhu1, 2, Fang Huang1, Liu Qin1, Wenguo Fan1, Hongwen He1 (1 Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong Province, China; 2 Huaihe Hospital, Henan University, Kaifeng, Henan Province, China) He YF, Zhu JH, Huang F, Qin L, Fan WG, He HG. Age-dependent loss of cholinergic neurons in learning and memory-related brain regions and impaired learning in SAMP8 mice with trigeminal nerve damage. Neural Regen Res. 2014;9(22):1985-1994. 文章全文请见：Neural Regen Res Age-dependent loss of cholinergic neurons and impaired learning in mice with TND The tooth belongs to the trigeminal sensory pathway. Dental damage has been associated with impairments in the central nervous system that may be mediated by injury to the trigeminal nerve. To confirm this, Dr. Yifan He, Guanghua School of Stomatology, Sun Yat-sen University, China established mouse models of Alzheimer’s disease and performed inferior alveolar nerve transection or sham surgery in middle-aged (4-month-old) or elderly (7-month-old) senescence-accelerated mouse prone 8 (SAMP8) mice. In the elderly group, animals that underwent nerve transection had fewer pyramidal neurons in the hippocampal CA1 and CA3 regions,