简并PCR技术及其在基因克隆中的应用.pdf

H EREDI TA S ( Beij ing) 25( 2) : 201~ 204, 2003 PCR 1, 2 1, 2 1 1 1 王洪振 , 周晓馥 , 宋朝霞 , 刘文广, 曾宪录 ( 1. , 130024; 2. , 136000) : PCR , , , PCR , PCR PCR , P CR , PCR P CR : P CR; ; : Q75 : A : 0253- 9772( 2003) 02- 0201- 04 Degenerate PCR and Its Application in Gene Cloning 1, 2 1, 2 1 1 1 WANG HongZh n , ZHOU XiaoFu , SONG ZhaoXia , LIU W nGuang , ZENG XianLu ( 1. Instit ute of Ge netics and Cell Biology , Northeast Nor mal Uni ersity , Changchu n 130024, China ; 2. Dep ar tment of Biology , Jilin Normal Uni ersity , Sip ing 136000, China) Abstract: D g n rat P CR is introduc d in this pap r, including w hat is d g n rat PCR, how to d sign d g n rat prim rs, how to optimiz d g n rat P CR param t rs, how to applying d g n rat PCR to obtain full- l ngth g n and which fi lds can apply d g n rat PCR. Th limits and r c nt advanc s of d g n rat PCR ar also discuss d. Bas d on this introduction, strat gi s of g n cloning and applications of d g n rat PCR in g n cloning ar summariz d in bri f. D g n rat PCR is a v ry us ful tool for s arching and discov ring n w g n s and n w m mb rs of a prot in family. Key words: d g n rat P CR; d g n rat prim r; g n cloning , , DNA , DNA , , DNA , , DNA PCR , G nBank PCR , PCR DNA , , , PCR , , , PCR , ( ) PCR 1. 1