Evaluationofhaemoglobin(erythrogen).pdfVIP

Plant Cell Rep (2004) 23:181–187 DOI 10.1007/s00299-004-0822-y C E L L B I O L O G Y A N D M O R P H O G E N E S I S M. Ganesan · N. Jayabalan Evaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L. cv. SVPR 2) Received: 7 July 2003 / Revised: 10 May 2004 / Accepted: 13 May 2004 / Published online: 24 July 2004 Springer-Verlag 2004 Abstract Somatic embryogenesis in cotton (Gossypium bin-mediated stress appeared to accelerate somatic em- hirsutum L.) is accelerated when the plant regeneration bryogenesis in cotton. medium is supplemented with haemoglobin (erythrogen). In cotton SVPR 2 lines, a higher frequency of embryoid Keywords Embryoids · Picloram · Antioxidant enzymes · formation was observed when the medium contained MS medium · Crop improvement 400 mg/l haemoglobin. Fresh weight of the callus, rate of embryoid induction, number of embryoids formed and the Abbreviations BAP: Benzylaminopurine · 2,4-D: percentage of plant regeneration from somatic embryos 2,4-Dichlorophenoxyacetic acid · GA3: Gibberellic acid · were increased. Among the two different cultivars tested, GR: Glutathione reductase · 2iP: Isopentenyl adenine · MCU 11 showed no response to the presence of hae- KT: Kinetin · NAA: Naphthaleneacetic acid · PFC: moglobin when compared to SVPR 2, and embryogenic Perfluorocarbon · PIC: Picloram · PO: Peroxidase · callus formation was completely absent in the former. ROS: Reactive oxygen species · SOD: Superoxide Medium containing MS salts, 100 mg/l myo-inositol, dismutase · T.HCl: Thiamine hydrochloride 0.3 mg/l thiamine-HCL, 0.3 mg/l Picloram (PIC), 0.1 mg/ l kinetin and 400 mg/l haemoglobin effected a better re- sponse with respect to embryogenic callus induction. Introduction After 8