《Ecology detection of moderate thermophilic enrichment at Lau Basin hydrothermal vents》.pdfVIP

J. Cent. South Univ. Technol. (2011) 18: 392−398 DOI: 10.1007/s11771−011−0709−3 Ecology detection of moderate thermophilic enrichment at Lau Basin hydrothermal vents 1 1 1 ZHOU Hong-bo(周洪波) , JI Hou-guo(姬厚国) , WEI Man-man(魏曼曼) , 1 2 WANG Yu-guang(王玉光) , CHEN Xin-hua(陈新华) 1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; 2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oeanography, State Ocean Administration, Xiamen 361005, China © Central South University Press and Springer-Verlag Berlin Heidelberg 2011 Abstract: Culturable thermophilic microorganisms were enriched from samples collected from Lau Basin hydrothermal vents in artificial seawater medium at 45 °C and pH 7.0. Microbial diversities of the enriched communities were defined by performing a restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences with enzymes MspI and Hin6 I. A total of 14 phylotypes have been detected by the RFLP patterns identified for 16S rRNA clone libraries of the enrichment. Analysis of sequences showed that at least four bacterial divisions presented in the clones libraries. The phyla Proteobacteria and Firmicutes were the most dominant groups. The majority of the sequences included in this analysis affiliated with Gamma Proteobacteria (71%) and Bacillus (23%). Scanning electron micrographs revealed that there were abundant rod