《Effects of Ginkgo biloba extract on expression of biomarkers during aflatoxin B1induced hepatocarcinogenesis in Wistar rats》.pdfVIP

Chinese-German Journal of Clinical Oncology May 2012, Vol. 11, No. 5, P261–P265 DOI 10.1007/s10330-012-0967-z Effects of Ginkgo biloba extract on expression of biomarkers during aflatoxin B1-induced hepatocarcinogenesis in Wistar rats 1 2 2 2 2 2 2 2 Yanrong Hao , Jianjia Su , Chao Ou , Ji Cao , Fang Yang , Xiaoxian Duan , Chun Yang , Yuan Li 1 Department of Radiotherapy, Clinical Cancer Center, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China 2 Cancer Institute of Guangxi Cancer Hospital, Guangxi Medicial University, Nanning 530021, China Received: 1 February 2012 / Revised: 5 March 2012 / Accepted: 25 March 2012 © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012 Abstract Objective: The aim of this study was to study the effect of Ginkgo biloba extract (EGb761) on metabolism of aflatoxin B (AFB ) in Wistar rats. Methods: Seventy one Wistar rats were assigned at random to groups A, B and C. Rats 1 1 in groups A, B were injected with AFB (intraperitoneal, 100–200 μg/kg body weight, 1–3 times/week). Group C was normal 1 control. Rats in group B were fed in food with EGb761, while rats in groups A, C were given normal food. Blood samples were collected and liver biopsies were performed on the 14th, 28th and 42nd week. All the rats were sacrificed on the 64th week. The incidence of hepatocarcinoma was investigated. The hepatic phase I drug-metabolizing enzyme Cytochrome-P450 (CYP450) and p