《EFFECTS OF MODERATE NOISE EXPOSURE ON HEARING FUNCTION IN C57BL6J MICE》.pdfVIP

20 14 Vol.9 No.2 JOURNAL OF OTOLOGY EFFECTS OF MODERATE NOISE EXPOSURE ON HEARING FUNCTION IN C57BL/6J MICE 1,3 1 1 2 1，2 SHI Chuang , SHI Lei , JIANG Xuejun , YANG Shiming , LIU Ke Abstract Objective To study characteristics of hearing loss after exposure to moderate noise exposure in C57BL/6J mice. Methods Male C57BL/6J mice with normal hearing at age of 5-6 weeks were chosen for this study. The mice were randomly selected to be studied immediately after exposure (Group P0), or 1 day (Group P 1), 3 days (Group P3), 7 days (Group P7) or 14 days (P14) after exposure. Their before exposure condition served as the normal control. All mice were exposed to a broad-band white noise at 100 dB SPL for 2 hours, ABR thresholds were used to estimate hearing status at each time point. Results ABR threshold elevation was seen at every tested frequency at P0 (P0.01). Elevation at high-frequencies ( 16 kHz and 32 kHz) was greater than at lower frequencies (4 kHz and 8 kHz, P0.05). From P 1 to P 14, ABR thresholds continuously improved, and there was no significant difference between P 14 and before exposure (P0.05). Conclusion There is a frequency specific re- sponse to 100 dB SPL broad-band white noise in C57BL/6J mice, with the high-frequency being more susceptible. Hearing loss induced by moderate noise exposure appears reversible in C57BL/6J mice. Key Words: C57BL/6J mice; Noise exposure; Hearing loss; Temporary noise-induced hearing loss; ABR threshold shift Nowadays, noise pollution has become a public row-band noise, exposure to moderate intensity broad- health problem[1-3] . Overexposure to noisy environment band environmental noise of variable time course can cause various kinds of dysfunction,