《Effects of the antitumor composition of the acetoacetate extract of vitex negundo seed on the growth of human cervical carcinoma xenografts in nude mice》.pdfVIP

Chinese-German J Clin Oncol March 2014, Vol. 13, No. 3, P105–P109 DOI 10.1007/s10330-013-1279-7 Effects of the anti-tumor composition of the acetoacetate extract of vitex negundo seed on the growth of human cervical carcinoma xenografts in nude mice* 1 1 2 3 1 4 Yanlin Cai , Zhongdong Chen , Aiqiong Tang , Bin Jiang , Zhaohua Fan , Jun Bai 1 Deparetment of Gynecolgy, The First Affiliated Hospital of University of South China, Hengyang 421001, China 2 Deparetment of Gynecolgy, Hunan Provincial Maternal and Child Health Hospital, Changsha 410008, China 3 Department of Plastic Surgery, The Second Affiliated Hospital of University of South China, Hengyang 421001, China 4 Deparetment of Obstetrics and Gynecolgy, Hangzhou Red Cross Hospital, Hangzhou 310003, China Received: 15 December 2013 / Revised: 17 January 2014 / Accepted: 14 February 2014 © Huazhong University of Science and Technology 2014 Abstract Objective: The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mice and its possible molecular mechanism. Methods: Models of human cervical cancer HeLa cells xenografts transplanted subcuta- neously in nude mice were established and randomly divided into 7 groups (each group including 5 nude mice): saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xe- nograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of LDH, ALT, Cr and WBC counts were examined and compared. The apoptot