《Effects of tirofiban on the reperfusionrelated noreflow in rats with acute myocardial infarction》.pdfVIP

Journal of Geriatric Cardiology (2013) 10: 52−58 ©2013 JGC All rights reserved; Research Article • Open Access • Effects of tirofiban on the reperfusion-related no-reflow in rats with acute myocardial infarction Xiao Liu, Gui-Zhou Tao Department of Cardiology, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China Abstract Objective To investigate the effects of tirofiban on the no-reflow phenomenon of acute myocardial infarction (AMI) rats received reperfusion, as well as the underlying mechanisms. Methods Fifty-six male Sprague-Dawley rats were randomly divided into four groups: Sham operation group (Sham), AMI/reperfusion group (AMI/R), Tirofiban group (Tiro) and Tiro+N-nitro-L-arginine group (L-NNA; an endothelial nitric oxide synthase inhibitor). To generate the animal model mimicking the no-reflow phenomenon, the rats first received oc- clusion of the left anterior descending coronary artery for 60 min and then followed by reperfusion for 120 min. Area of no-reflow, area at risk and area of necrosis were measured by thioflavine S, Evans blue and triphenyl tetrazolium chloride staining, respectively. Haemody- namic function was measured at the end. In the meantime, nitric oxide synthase (NOS) activity was determined by a NOS assay kit. The expression of myocardial endothelial nitric oxide synthase (eNOS) was determined by an enzyme-linked immunosorbent assay (ELISA). The 1177 1177 expression of phosphorylated eNOS at Ser (p-eNOS Ser ) and vascular endothelial-cadherin (VE-cadherin) were determined by west- ern blot. Results Compared with AMI/R group, tirofiban significantly reduced th