《Effect of Tiantai No1天泰1号 on βAmyloidinduced Neurotoxicity and NF к B and cAMP Responsive Elementbinding Protein》.pdfVIP
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·286·
EXPERIMENTALRESEARCH
Tiantai
No.1(天秦1号)on13·Amyloid—induced
andNFmKBandcAMPRes
wu de LI
Vellis3,and
Zheng-zhi(吴正治)1,AndrewC.J.Huan92,Jean Ying—hong(李映红)1
theeffectandmolecularmechanismofTiantai
ABSTRACTObjective:To No.1(天泰1号),a
investigate
C:hineseherbaI the andreductionof inducedbeta-
neurotoxicity
compound preparation.forprevention by
vitroanditseffectsonnuclearfactor-KF—K cAMP
amyloidpeptides(Abeta)fn B(NB)and responsive
the neuronal
transfection 04
element-bindingprotein(CREB)pathwaysusinggene technique.Methods:B1
the TiantajNo.1On the Abeta.The
cellswereusedtoexamineeffectsof jnduced
Ioweringneurotoxicityby
cellswere withTiantaiNo.1atdosesof for3
50,100,150,or respectivelydays
pre-treated 200¨g/mL
and withTiantaiNo.1 48h or
co-treated and 131-40,10¨mol/L)for
beta·amyloidpeptidel一40(A post-
treatedwithTiantaiNo.1for48hafterthecellswere to 25-35)
exposedbeta-amyloidpeptides25-35(A13
for8h.In transfection weretreatedwithTiantaiNo.1at and
g
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