《Effects of oral administration recombinant Saccharomyces cerevisiae expressing humansalmon calcitonin on hypercalcemia rats》.pdfVIP
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HIGHTECHNOLOGYLETFERSIV01.18No.1IMar.2012
Ipp.107—111
doi:10.3772/j.issn.1006—6748.2012.01.018
Effectsoforaladministrationrecombinant cerevisiae
Saccharomyces
human.salmoncalcitoninon rats①
expressing hypercalcemia
Y。ng(姜勇)…,zhangxuechen产+,sllIlPin印an…,ChenYuns。ng’
Jiang
ofMarineLife
Sciences,OceanUniversity 266003,P.R.China)
(+College ofChina,Qingdao
(”NationalOceanographicCenter,Qingdao266071,P.R.China)
ofShantou 51 507
(”+MedicalCollege University,Shantou1,P.R.China)
Abstract
and ontheeellsurfacesof
Recombinanthuman.salmoncalcitonin(hsCT)was
synthesizedexpressed
recombinant the of
Saccharomycescerevisiae(yAGA2一hsCT)usingpAGA2一hsCTexpressionvector.Expression
wasverifiedflow The ofosteoclastswasinhibited
recombinanthsCT cytometer by
by resorptionactivity
recombinanthsCT whichreleasedfromtheeellsurfaceof Bloodcalciumdeterminationin
protein yAGA2-hsCT
ratsshowedthe oraladministrationof5 transformantto
activityofyAGA2·hsCTfollowing lyophilized yeast
m班g
the from mMto2.662土0.012mM.
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