《Histological assessment in peripheral nerve tissue engineering》.pdfVIP

NEURAL REGENERATION RESEARCH September 2014,Volume 9,Issue 18 INVITED REVIEW Histological assessment in peripheral nerve tissue engineering 1, 2 1 1 2 Víctor Carriel , Ingrid Garzón , Miguel Alaminos , Maria Cornelissen 1 Department of Histology (Tissue Engineering Group), Faculty of Medicine, University of Granada, and Instituto de Investigación Biosanitaria ibs, Granada, Spain 2 Department of Basic Medical Sciences (Histology and Tissue Engineering Group), Faculty of Medicine, Ghent University, Ghent, Belgium Abstract Corresponding author: The histological analysis of peripheral nerve regeneration is one of the most used methods to Víctor Carriel, M.Sc., M.Biol., Ph.D., demonstrate the success of the regeneration through nerve conduits. Nowadays, it is possible Department of Histology and Tissue to evaluate different parameters of nerve regeneration by using histological, histochemical, Engineering Group (CTS-115), Faculty immunohistochemical and ultrastructural techniques. The histochemical methods are very of Medicine, University of Granada, Av. sensible and are useful tools to evaluate the extracellular matrix remodeling and the myelin de Madrid 11, 18012 Granada, Spain; sheath, but they are poorly specific. In contrast, the immunohistochemical methods are highly Department of Basic Medical Sciences specific and are frequently used for the identification of the regenerated axons, Schwann cells (Histology, Tissue Engineering Group), and proteins associated to nerve regeneration or neural linage. The ultrastructural techniques Faculty of Medicine, Ghent University, offer the possibility to perform a high resolution morphological and quantitative analysis of De Pint