《HSP27在左侧结肠癌和右侧结肠癌差异表达的实验研究》.pdfVIP

Chinese-German Journal of Clinical Oncology April 2011, Vol. 10, No. 4, P214–P219 DOI 10.1007/s10330-011-0780-0 HSP27: a candidate differentially expressed protein between left- and right-sided colon carcinomas* 1 2 3 2 3 Liang Zeng , Hong Zhu , Haiping Pei , Li Liu , Linsheng Huang 1 Department of Pathology, Hunan Tumor Hospital, Changsha 410013, China 2 Department of Clinical Oncology, Xiangya Hospital, Central-South University, Changsha 410008, China 3 Department of Surgery, Xiangya Hospital, Central-South University, Changsha 410008, China Received: 30 December 2010 / Revised: 25 January 2011 / Accepted: 15 March 2011 © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2011 Abstract Objective: The aim of the study was to screen differentially expressed proteins between left- and right-sided colon cancers by proteomics techniques and provide molecular genetic basis for oncobiological difference between left- and right- sided colon cancers. Methods: Tissue samples including left- and right-sided colon cancers were collected and preserved in the –80 ℃ refrigeratory. In the first part of our experiment, protein separating was performed by using two-dimensional gel electrophoresis (2-DE) and the images of the gels were acquired by the scanner and then analyzed to find the differentially expression protein-spots in different groups. The peptide mass fingerprintings (PMF) was acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and the proteins were identified by data searching in the Mascot-database. Differentially expression proteins were assayed by RT-PCR, Western blot, and immun