BCA蛋白定量试剂盒(Thermo)使用指南.pdf

INSTRUCTIONS Pierce® BCA Protein Assay Kit 23225 23227 1296.7 Number Description 23225 Pierce BCA Protein Assay Kit, sufficient reagents for 500 test-tube or 5000 microplate assays 23227 Pierce BCA Protein Assay Kit, sufficient reagents for 250 test-tube or 2500 microplate assays Kit Contents: BCA Reagent A, 1000mL (in Product No. 23225) or 500mL (in Product No. 23227), containing sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartrate in 0.1M sodium hydroxide BCA Reagent B, 25mL, containing 4% cupric sulfate Albumin Standard Ampules, 2mg/mL, 10 ×1mL ampules, containing bovine serum albumin (BSA) at 2mg/mL in 0.9% saline and 0.05% sodium azide Storage: Upon receipt store at room temperature. Product shipped at ambient temperature. Note: If either Reagent A or Reagent B precipitates upon shipping in cold weather or during long-term storage, dissolve precipitates by gently warming and stirring solution. Discard any kit reagent that shows discoloration or evidence of microbial contamination. Table of Contents Introduction 1 Preparation of Standards and Working Reagent (required for both assay procedures)2 Test Tube Procedure (Sample to WR ratio = 1:20) 3 Microplate Procedure (Sample to WR ratio = 1:8) 3 Troubleshooting 4 Related Thermo Scientific Products 5 Additional Information 5 References 6 Introduction The Thermo Scientific Pierce BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the color