变性梯度凝胶电泳及其在土壤微生物生态学中的应用.pdfVIP

中国农学通报 2011,27(03):6-9 Chinese Agricultural Science Bulletin 变性梯度凝胶电泳及其在土壤微生物生态学中的应用 李 丹，王秋玉 （东北林业大学生命科学学院，哈尔滨150040） 摘 要：变性梯度凝胶电泳（denaturing gradient gel electrophoresis ，DGGE ）因其具有可靠性强、易操作、可 同时分析多个样品等优点，目前已经成为一种普遍的快速指纹分析分子技术，广泛应用于微生物群落组 成、多样性及种群动态等研究。笔者综述了DGGE 技术的基本原理和发展，以及该技术在土壤微生物 生态学领域诸多方面的应用，并进一步对该技术在微生物生态学领域研究中存在的优势和局限性进行 了讨论评价，最后对其应用前景进行了展望。 关键词：DGGE ；微生物群落结构；多样性；土壤微生物生态学 中图分类号：Q933 文献标志码：A 论文编号：2010-2986 DenaturingGradientGelElectrophoresisanditsApplicationinSoilMicrobialEcology Li Dan, Wang Qiuyu (CollegeofLifeSciences, NortheastForestryUniversity, Harbin 150040) Abstract: Denaturing gradient gel electrophoresis (DGGE), which has advantages of high reliability, easy operation, and multiple samples to be processed simultaneously, has become a commonly used molecular technique for rapid fingerprint analysis in microbial community composition, diversity, population dynamics study and so on. Here, a background to the theory and progress of DGGE and its application in soil microbial ecological aspects were reviewed. Furthermore, the advantages and limitations of this technique in the study on microbial ecology were discussed and evaluated. Finally, its application potential was prospected. Keywords:DGGE; microbial community structure; diversity; soil microbial ecology [7] 0 引言 统中复杂的微生物群体将导致极大的偏差 。近几十 土壤在维持地球生态系统平衡上起着重要作用， 年发展起来的分子生物学技术，弥补了传统微生物培 但是由于人类对土地资源的不当管理与利用，土壤质 养和鉴定方法不能真实反映待测环境的不足。这些群 [1]