HKC细胞损伤前后对草酸钙晶体吞噬能力的差异.pdf

第26拯第12期 无 机 化 学 学 报 V01．26No．12 INOR(,ANIC 2010qi12月 CHINESEJOURNAl。()}1 CHEMISrI’RY 2131．2137 HKC细胞损伤前后对草酸钙晶体吞噬能力的差异 谈金 邓穗平 欧阳健明★ (暨南大学生物矿化与结石病防治研究所，暨南大学化学系，广州 510632) 晶体的成核和聚集．从而增加了肾结石形成的危险性。 关键词：草酸钙；细胞调控；肾结石；生物矿化 中图分类号：R69；R329；0614．23+1文献标识码：A 文章编号：100124861(2010)12．2131-07 Differenceof toCalciumOxalate Endocytosis Crystalsby HKCCellsbeforeandafter Injury TAN DENG OUYANG Jin Sui-Ping Jian-Ming+ Biomineralizationand 51 (Institute Lithias蠡Research,．厍nanUniversity,Guangzhou0632) of of tubule usedto Abstract：Anoxidativemodelhuman injury kidneyproximalepithelial beforeandafter thedifferenceof ofcalcium HKC oxalate(CaOxa)modulatedinjury． investigate crystallization by Cell wasexamined Cell of of viability byusing CountingKit(CCK-8)assay；thechangeexpressioncrystal adherentmolecule HKCafter Wasobservedlaserconfocal osteopontin(OPN)oninjury by ofHKCwagobservedinverted microstruetumofHKCandthe morphologicalchange by microscope；the crystals HKCwereobservedmeansof electron w鹪 induced scanning microscopyrSEM)；thecrystalcomponent by by characterized oxalate