siRNA干扰Survivin基因对结肠癌生物学特性影响.pdfVIP

科技导报201 针塾 研究论文(Articles) 1。29(07) si 生物学特性影响 王晓春。文贤慧 中南大学湘雅医学院医学检验系．长沙410013 细胞。转染24，48，72h后．3组Survivin蛋白表达均出现下调(PO．05J。在48h时达到顶峰。抑制率分别为66．5％±2．1％。49．6％± 的体外Survivin 保守区域设计的靶向siRNA。干扰效果更为显著。 ． 关■调Survivin；基因沉默：siRNA；M丌法；细胞生物学特性 中圈分类号R735．3 文t标识码A doi10．3981力．issn．1000—7857．2011．07．002 Influenceof siRNAon Features Survivin the Gene-targetingBiological ofColorectalCarcinomaCells WANG Xianhui Xiaochun，WEN Medicd South DepartmentofLaboratoryMedicine，XiangyaSchool,CentralUniversity,Changsha410013,China Ab嘲瞅SurvivinsiRNAistransfectedtocoloncancerceHsSW480andtheinterferenceon and isstudiedto proliferation apeptosis the basisfor ofcoloncancerandseek RNAinterference siRNA provideexperimentalgenetherapy new，effective fragment．Three SurvivinwereconstructedandtransfectedintoSW480．After ofsurvivin Wasdetected targeting gene 24，48，and72h，the change protein siRNA Westerninthesame Wasdetected MTY resultsshowthatafterthree byusing b104 time，cell byusing assay．The proliferation effect all onSW480cellsat ofSurvivin are the 24，48，and72h，threegroups proteinexpressionappeared 48hand ofSurvivin reached