《Microbial community structure and nitrogenase gene diversity of sediment from a deepsea hydrothermal vent field on the Southwest Indian Ridge》.pdfVIP

Acta Oceanol. Sin., 2014, Vol. 33, No. 10, P. 94–104 DOI: 10.1007/s13131-014-0544-0 E-mail: hyxbe@263.net Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge 1§ 2§ 1 3 4,5 1 WU Yuehong , CAO Yi , WANG Chunsheng , WU Min , AHARON Oren , XU Xuewei * 1 Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, China 2 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China 3 College of Life Sciences, Zhejiang University, Hangzhou 310058, China 4 Department of Plant and Environmental Sciences, the Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel 5 The Hebrew University of Jerusalem, The Edmond J. Safra Campus, Givat Ram, Jerusalem 91904, Israel Received 25 March 2013; accepted 2 November 2013 ©The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2014 Abstract A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction (PCR) amplification of the 16S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E (MBGE) and marine group I (MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria , Bacteroidetes, Planctomycetes, Acidobacte