《Lipopolysaccharide enhances the inhibition of NFκB expression in NNKmediated peritoneal macrophages》.pdfVIP

Chinese-German J Clin Oncol July 2014, Vol. 13, No. 7, P332–P336 DOI 10.1007/s10330-014-1322-3 Lipopolysaccharide enhances the inhibition of NF-κB expression in NNK-mediated peritoneal macrophages 1 2 1 Bin Li , Mei Wu , Xiaoping Liu 1 Department of Histology and Embryology, Medical College of Qingdao University, Qingdao 266071, China 2 Laboratory of Human Microscopic Structure, Experimental Center of Basic Medicine, Medical College of Qingdao University, Qingdao 266071, China Received: 22 April 2014 / Revised: 17 May 2014 / Accepted: 5 June 2014 © Huazhong University of Science and Technology 2014 Abstract Objective: The aim of the study was to investigate the effect of lipopolysaccharide (LPS) on the expression of nuclear factor kappa B (NF-κB) in 4-(methylitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-mediated primary mouse peritoneal macrophages in vitro. Methods: The activity of peritoneal macrophages treated with different concentrations of LPS was de- tected by MTT assay in rider to find the optimal concentration. Peritoneal macrophages were also treated with NNK (100–500 μM), with or without LPS for 9 h. The expression of NF-κB was demonstrated via immunocytochemistry (ICC) and Western- blot, respectively. Results: The concentration of LPS at 25 μg/mL was found to be the optimal concentration to improve the activity of peritoneal macrophages (P 0.01). Simultaneously, LPS (25 μg/mL) increased the expression of NF-κB in both the nucleus and cytoplasm and facilitated transfer of NF-κB to the nucleus. NNK treatment significantly inhibited the expression of NF-κB in a concentration-dependent manner, among the LPS-stimulated or unstimulated peritoneal macrophages, espe-