《Longterm treatment with PP2 after spinal cord injur y resulted in functional locomotor recover y and increased spared tissue》.pdfVIP

NEURAL REGENERATION RESEARCH December 2014,Volume 9,Issue 24 Long-term treatment with PP2 after spinal cord injury resulted in functional locomotor recovery and increased spared tissue 1 1 2 1 1 1 Odrick R. Rosas , Aranza I. Torrado , Jose M. Santiago , Ana E. Rodriguez , Iris K. Salgado , Jorge D. Miranda 1 Department of Physiology, School of Medicine, University of Puerto Rico Medical Sciences Campus, San Juan, PR, USA 2 Department of Natural Sciences, University of Puerto Rico Carolina Campus, Carolina, PR, USA Abstract Corresponding author: The spinal cord has the ability to regenerate but the microenvironment generated after trauma Jorge D. Miranda, Ph.D., Physiology reduces that capacity. An increase in Src family kinase (SFK) activity has been implicated in Department, University of Puerto neuropathological conditions associated with central nervous system trauma. Therefore, we Rico School of Medicine, San Juan, PR hypothesized that a decrease in SFK activation by a long-term treatment with 4-amino-5-(4- 00936-5067, USA, chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyramidine (PP2), a selective SFK inhibitor, after jorge.miranda3@. spinal cord contusion with the New York University (NYU) impactor device would generate a permissive environment that improves axonal sprouting and/or behavioral activity. Results doi: 10.4103/1673-5374.147949 demonstrated that long-term blockade of SFK activation with PP2 increases locomotor activity / at 7, 14, 21 and 28 days post-injury in the Basso, Beattie, and Bresnahan open field test, round