Chemical Composition of Hexane Extract of Citrus aurantifolia.pdfVIP

Molecules 2012, 17, 11173-11184; doi:10.3390/molecules1座机电话号码 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Chemical Composition of Hexane Extract of Citrus aurantifolia and Anti-Mycobacterium tuberculosis Activity of Some of Its Constituents Nallely E. Sandoval-Montemayor 1, Abraham García 1, Elizabeth Elizondo-Trevi?o 1, Elvira Garza-González 2, Laura Alvarez 3 and María del Rayo Camacho-Corona 1,* 1 Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza CP 66451, Nuevo León, Mexico 2 Facultad de Medicina, Universidad Autónoma de Nuevo León, Madero y Aguirre Peque?o, Mitras Centro, Monterrey CP 64460, Nuevo León, Mexico 3 Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca CP 62209, Morelos, Mexico * Author to whom correspondence should be addressed; E-Mail: maria.camachocn@.mx; Tel.: +52-81-8329-4000 ext. 3463 ; Fax: +52-81-8352-9025. Received: 10 August 2012; in revised form: 12 September 2012 / Accepted: 12 September 2012 / Published: 19 September 2012 Abstract: The main aim of this study was to isolate and characterize the active compounds from the hexane extract of the fruit peels of Citrus aurantiifolia, which showed activity against one sensitive and three monoresistant isoniazid, streptomycin or ethambutol strains of Mycobacterium tuberculosis H37Rv. The active extract was fractionated by column chromatography, yielding the following major compounds: 5-geranyloxypsoralen 1 ; 5-geranyloxy-7-methoxycoumarin 2 ; 5,7-dimethoxycoumarin 3 ; 5-methoxypsoralen 4 ; and 5,8-dimethoxypsoralen 5 . The structures of these compounds were elucidated by 1D and 2D NMR spectroscopy. In addition, GC-MS analysis of the hexane extract allowed the identification of 44 volatile compounds, being 5,7-dimethoxycouma