《2_ftp》.pdf

DEVELOPMENTAL GENETICS 19~290-301(1996) Origin of Primordial Germ Cells in the Prestreak Chick Embryo L. KARAGENC, Y. CINNAMONF M. GINSBURG,2 AND J.N. PETITTEI Department of Poultry Science, North Carolina State University,Raleigh, North Carolina 2Departmentof Cell and Animal Biology, Silberman Institute ofLifeSciences, Hebrew University of Jerusalem, Jerusalem, Israel I ABSTRACT The temporal and spatial pattern of biologists for almost a century. This long history of segregation of the avian germline from the formation of attention is well deserved, since germ cells provide the area peiiucido to the beginning of primitive streak genetic continuity from generation to generation, a formation (stagesVll-XlV, EGBK)was investigated using feature which makes them the cell type responsible not the culture of whole embryos and central and peripheral only for perpetuation of the speciesbut also the biologi- embryo fragments on vitelline membranes at stages cal vehicle for evolutionary change. In all vertebrates, VII-IX, immunohistological analysis of whole mount em- primordial germ cells (PGCs) appear during early bryos and sections with monoclonal antibodies MC-480 stages of development in extragonadal sites; yet, their against stage-specific embryonic antigen-1 (SSEA-1) mode of development has been clarified only in an- and E M - 1 , and with the culture of dispersed blast0 urans, urodales [see Nieuwkoop and Sutasurya, 19791 derms at stages IX-XIV with and without an STO feeder and more recently in the mouse [Eddy et al., 1981; layer. Whole embryos at intrauterine