《chapter10 Homologous recombination at the molecular level》.ppt

Welcome Each of You to My Molecular Biology Class CHAPTER 10 Homologous recombination at the molecular level All DNA is recombinant DNA. Genetic exchange works constantly to blend and rearrange chromosomes, most obviously during meiosis, when homologous chromosomes pair prior to the first nuclear division. During this pairing, genetic exchange between the chromosomes occurs. This exchange, classically termed crossing over, is one of the results of homologous recombination. OUTLINE Models for Homologous Recombination (two models)** Homologous Recombination Protein Machines (RecBCD pathway)** Homologous Recombination in Eukaryotes (meiosis recombination, proteins) Mating-Type Switching (a gene conversion event) Genetic Consequences of the Mechanism of Homologous Recombination (exchange or conversion) * * Molecular Biology of the Gene, 5/E --- Watson et al. (2004) Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods 3/29/05 Part II: Maintenance of the Genome Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next Ch 6: The structures of DNA and RNA Ch 7: Chromosomes, chromatins and the nucleosome Ch 8: The replication of DNA Ch 9: The mutability and repair of DNA Ch 10: Homologous recombination at the molecular level Ch 11: Site-specific recombination and transposition of DNA 3/29/05 Different changes of DNA sequences Listen to the nature Mutation is bad and naturally repaired, thus it could not be responsible for biodiversity. Recombination is good and naturally promoted; it is responsible for diversity inside of species. Transposition is different from mutation and recombination because (1) producing mechanism is different; (2) no mechanism to correct it; (3) existing in nature in a well-controlled manner (10-5). Not repaired but controlled. Molecular Biology Course Topic 1: Models for Homologous Recombin