《Natural variation in GS5 plays an important role in regulating grain size and yield in rice》.pdf

l e t t e r s Natural variation in GS5 plays an important role in regulating grain size and yield in rice Yibo Li, Chuchuan Fan, Yongzhong Xing, Yunhe Jiang, Lijun Luo, Liang Sun, Di Shao, Chunjue Xu, Xianghua Li, Jinghua Xiao, Yuqing He Qifa Zhang Increasing crop yield is one of the most important goals of in the summer of 2006 in Wuhan (for example, see Supplementary . plant science research. Grain size is a major determinant of Table 2). Using this data, GS5 was mapped between C35 and RM574 d e grain yield in cereals and is a target trait for both domestication (Fig. 1b). Analysis of population 2 detected 15 recombinants between v r  e and artificial breeding . We showed that the quantitative C35 and RM574 (Fig. 1c). In total, 10 recombinant plants were found s trait locus (QTL) GS5 in rice controls grain size by regulating in the two populations in the interval between S2 and RM574. However, e r s grain width, filling and weight. GS5 encodes a putative serine progeny testing revealed an inconsistency for three individuals between t h carboxypeptidase and functions as a positive regulator of grain the marker genotypes determined using C62, which is located in the g i r size, such that higher expression of GS5 is correlated with middle of the GS5 region, and the genotype deduced by progeny test- l l larger grain size. Sequencing of the promoter region in ing (nos. 30, 8396 and 57-5 in Fig. 1d and Supplementary Table 2). A . 5 rice accessions from a wide geographic range identified By referencing the previously cloned qSW5/GW5