低表达甘蓝花药发育相关基因小体积荧光定量反应体系的建立和可靠性分析.pdfVIP

20 11, 20(4) : 111- 115 Acta Agriculturae Boreali-occidentalis Sinica 低表达甘蓝花药发育相关基因小体积荧光定量 * 反应体系的建立和可靠性分 1, 2 2 1 2 王效维 , 刘海霞 , 杨颖丽, 康俊根 ( 1. , 730070, ; 2. , 100097) : qRT- PCR , A T-H OOK , , , 10 LL , , : ; ; ; P CR ; : S635 : A : 1004- 1389( 20 11) 04-0111-05 A Rea-l time Quantitative PCR Protocol with Small Reaction Volume for Studies of Low Expression Genes Related to Cabba e Anther Development 1, 2 2 1 2 WA NG Xi ow ei , LIU H ix i , YANG Yingli nd KANG Jungen ( 1. Northw est Norm l University, L nzh ou 730070, Chin ; 2. Beijing Veget ble Rese r ch Center of BAA FS , Beijing 100097, Ch in ) Abstract: M ny import nt genes h ve rel tively low expr ession level during pl nt development . T o est blish che p nd reli ble qu ntit tive PCR protocol for expression studies of low-copy-number genes, w e used the low expression gene A T-H OOK in c bb ge nther to ev lu te the fe sibility of re l-time qu ntit tive PCR w ith sm ll volume reg rding the st bility nd efficiency . A fe sible 10 LL re l-time qu ntit tive PCR protocol w s est blished. T he exper iment here show ed th t the sm ll vol- ume qu ntit tive PCR sy stem w s st ble nd reli ble nd it could be helpful for the extensive expr es- sion studies of low-copy-number genes rel ted to c bb ge nther development . Key words: Brassica oleracea; Gene expression; Sm ll volume re ction system; Re -l time qu ntit tive PCR; Fe sibility , [ 1] ,