补骨脂对激素性股骨头坏死骨小梁面积分数和空骨陷窝影响的实验的研究.pdfVIP

摘要 中文摘要 目的：以马血清加甲强龙造模激素性股骨头坏死模型为研究对象，研究补骨脂治 疗激素性股骨头坏死的初步效果和作用机理，为其临床应用提供理论依据。 方法：选用日本大耳白兔30 只，体重分别为 1.5～2kg，由河南省动物实验中心 提供。Matsui 提出的马血清+甲基强的松龙建立激素性股骨头坏死兔模型，分为 空白组、模型组、治疗组三组进行比较。造模完成后，空白组每天用生理盐水 10ml 灌胃一次，模型组每天用生理盐水 10ml 灌胃一次，治疗组用补骨脂提取液 10ml 灌胃一次。8 周后，解剖出股骨头并观察股骨头形态，色泽，脱钙，包埋， 切片，HE 染色，光镜下检测各组骨小梁面积及骨陷窝数，公式计算骨小梁面积 分数及空骨陷窝比率，并进行不同组间比较。 结果：本实验中光镜下可见空白组和治疗组骨小梁面积分数显著高于模型组，空 缺骨陷窝率显著低于模型组，差异显著（P 0.05），可见补骨脂能够促进股骨 头骨组织再生,减少空骨陷窝数,以达到防治骨质疏松的作用，进一步达到治疗股 骨头坏死，延缓塌陷的目的。 结论：大量使用激素后能够导致骨小梁变细, 结构紊乱，甚至部分断裂，空缺骨 陷窝数明显增多；中药补骨脂能够显著提高骨小梁面积分数，降低空骨陷窝数， 对补骨脂治疗激素性股骨头坏死提供初步理论依据。 关键词：激素性股骨头坏死；补骨脂；现代治疗；实验研究 1 补骨脂对激素性股骨头坏死骨小梁面积分数和空骨陷窝影响的实验研究 The psoralen femoral head necrosis of trabecular bone area fraction and empty bone lacunae impact experimental study Abstract Objective: To horse serum plus methylprednisolone modeling of femoral head necrosis model for the study, psoralen treatment of femoral head necrosis initial effect and mechanism of action, provide a theoretical basis for its clinical application. Methods: 30 Japanese white rabbits, weighing 1～1.5kg, were provided by the Animal Experimental Center of Henan Province. Matsui horse serum + methyl prednisolone femoral head necrosis rabbit model divided into control group, model group, treatment group compared with the three groups. Modeling is complete, the blank group with saline 10ml a day orally once a model group orally once a day with saline (10 ml), the treatment group with psoralen extract 10ml orally once. After 8 weeks, the anatomical femoral head and to observe the shape of the femoral head, color, decalcified, embedded, sliced, HE staining, light microscopic detection of each group of trabecular bone area and bone lacuna formula trabecular bone area fraction and of empty lacunae ratio, and comparison between differen