酿酒酵母固态发酵白酒糟生产蛋白饲料的研究_张轩.pdfVIP

《饲料工业》·2012 年第33 卷第19 期 试验研究 酿酒酵母固态发酵白酒糟生产蛋白饲料的研究 张 轩 赵述淼 陈海燕 梁运祥 (华中农业大学楚天学院，湖北武汉 430205) 摘 要：以廉价的白酒糟为主要基质，接种酿酒酵母，开展酵母高密度固体发酵的研究。最终确定 了在实验室条件下以白酒糟为基质进行酵母高密度固体发酵的工艺流程为：250 ml 三角瓶装入干白 酒糟 10 g，并补加混合营养液2 ml （以干糟质量的8%添加糖蜜，以0.5 gN/kg 干糟的量添加尿素），摇 匀分散，加入干糟质量4% 的复合酶制剂，调整酒糟的含水量为45%~50% ，按0.7 亿/g 干糟接种后于 30 ℃恒温箱中发酵培养72 h ，酵母总数约30 亿/g 干糟，烘干粉碎即可制得酵母蛋白饲料。 关键词：白酒糟；酵母；高密度固体发酵；蛋白饲料 中图分类号： 文献标识码： 文章编号 S816.4 A ：1001-991X （2012）19-0027-05 Study on producing protein feed through the solid state fermentation of distillers grains with saccharomyces cerevisiae Zhang Xuan, Zhao Shumiao, Chen Haiyan, Liang Yunxiang ： Abstract We use low-cost distillers grains as main substrate and inoculate saccharomyces cerevisiae, by adopting yeast density solid state fermentation, we find the laboratorial technological process of yeast density solid state fermentation using distillers grains as the substrate: 10 g distillers dried grains is put into 250 ml conical flask, adding 2 ml nutrient solution to the substrate (add molasses which occupying 8% of the quality of distillers dried grains and urea which has 0.5 gN/kg distillers dried grains), shake up and disperse ，adding dedicated enzymic preparations for white distillers grains fermentation which occupying 4% of the quality of distillers dried grains, inoculum 0.7 million activated yeast per kilogram distillers dried grains, adjust the water content of distillers grains to 45%~50%,take the flasks into 30 ℃ incubator to cultivate after sealing , at intervals of 12 h reverse the ma