单克隆抗体与多克隆抗体配对ELISA方法比较.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGYBULLETIN 2009年第11期 单克隆抗体与多克隆抗体配对ELISA方法比较 张小兵1 邸禄芹2 吴萌1 闫静辉1 (1河北省生物研究所，石家庄050081；2河北医科大学第三医院，石家庄050051) 摘要： 以人绒毛膜促性腺激素(HCG)为抗原，制备出对HCG的多克隆抗体和特异性单克隆抗体，并进行抗体纯化和 特性分析，利用辣根过氧化物酶(HRP)分别对其进行了标记。采用双抗夹心ELISA试验，探讨了多克隆抗体与单克隆抗体配 对的若干事项。结果表明，利用单克隆抗体和酶标多克隆抗体配对，并用含动物血清的稀释液稀释酶标抗体，可实现对检测 原的高特异性和高灵敏度检测。 关键词：·单克隆抗体多克隆抗体抗体配对ELISA法 ofthe of Antibodiesand Study OptimumPairingPolyclonal Antibodieswith ELISA Monoclonal Sandwich Di Wu Yan ZhangXiaobin91Luqin2 Men91 Jinghuil Institute 05008 (1Biology o，Hebei，Shijiaahuang1； 2 TheThird Medical HospitalofHebei University，Shijiazhuang050051) humanchorionic the antibodiesandmonoelonal Abstract：Using gonadotrophin(HCG)asantigen，polyclonal with forHCGwere and theseantibodieswerelabeledhorseradish specific generated，characterizedhighlypurified．Then peroxidase adouble sandwich immunosorhent ofthe of (HRP)．By antibody enzyme-linked assay，severalproblemsoptimumpairingpolyelonal舳一 tibodiesandmonoclonalantibodieswerediscussed．Theresultsshowedthatwithmonoclonalas and antibodytrappingantibodypoly- as ELISAhada clonal with as whichshouldbedilutedwithanimalseradiluent，thesandwich antibodylabelingenzymetestingantibody and todetect