褪黑素对大鼠肾脏缺血再灌注损伤的保护作用.docVIP

褪黑素对大鼠肾脏缺血再灌注损伤的保护作用 　　作者：杜娟 白光辉 作者单位：青海大学医学院 　　【摘要】 目的 探讨褪黑素(melatonin,Mel)对大鼠缺血再灌注(IR)损伤的保护作用及机制。方法 通过手术建立大鼠肾缺血再灌注损伤模型，将实验动物随机分为Sham组、IR组和Mel+IR组，测IR后24 h各组大鼠血清中尿素氮(BUN)和肌酐(Cr)、肾组织匀浆中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量变化，并观察肾脏组织结构的病理变化。结果 IR后24 h大鼠血清BUN、Cr水平明显升高，组织匀浆SOD活力明显降低，MDA水平明显升高。Mel+IR组较IR组肾脏SOD活性明显升高(P0.01)，MDA水平明显降低(P0.01);肾功能、组织变化较IR组得到了明显改善。结论 褪黑素可通过提高组织抗氧化酶活性、降低组织脂质过氧化反应发挥对肾功能及肾脏结构的保护作用。 杜娟(1980～)，女，汉族，河南籍，2005级肾脏病学专业研究生 　　【关键词】 褪黑素 缺血再灌注损伤 超氧化物歧化酶 丙二醛 　　THE PROTECTIVE EFFECT OF MELATONIN ON RENAL 　　ISCHEMIA REPERFUSION(IR) INJURY IN RAT 　　Du juan 1, Bai Guanghui 2 　　(1.QingHai University Medical College; 　　2.Department of Nephrology，Affiliated Hospital of QingHai University ) 　　Abstract Objective To investigate the protective effect and mechanism of melatonin on renal ischemia/reperfusion(IR) injury in rat . Methods Using an in vivo rat model of IR, we observed the change of BUN and Cr in blood serum, and changes of the activities of superoxide dismutase (SOD), and malondiadehyde (MDA) in homogenized kidney tissue, and tissue structural pathological change during 24h after reperfusion. Results The level of BUN and Cr in blood serum and MDA in homogenized kidney tissue production was significantly increased and the level of activities of SOD production was significantly decreased. The level of BUN and Cr and MDA in the Mel-treated rat was lower (P0.01), and the level of activities of SOD was higher (P0.01) than IR group. Also the Mel-treated rats pathological change showed significant improvement during 24h after reperfusion. Conclusion Melatonin can protect renal function and tissue structure of IR injury through improving the activities of antioxidase and decreasing tissue lipid peroxidation. 　　Key words Melatonin ischemia/reperfusion injury superoxide dismutase malondialdehyde 　　了解肾缺血再灌注损伤( IRI) 的发病机制及寻找更多更有效的药物防治肾IRI，是本学科领域的一个重要问题。本实验试图了解当肾脏发生缺血再灌注损伤时褪黑素对其的保护机制。 　　1 材料与方法 　　1.1 材料 　　褪黑素(Mel)购自美国Sigma公司， BU