2011-美国BerndKubista-基因芯片分析不同组织亚型骨肉瘤基因差异性表达.pdfVIP

International Orthopaedics (SICOT) (2011) 35:401–411 DOI 10.1007/s00264-010-0996-6 ORIGINAL PAPER Microarray analysis identifies distinct gene expression profiles associated with histological subtype in human osteosarcoma Bernd Kubista Florian Klinglmueller Martin Bilban Martin Pfeiffer Richard Lass Alexander Giurea Phillipp T. Funovics Cyril Toma Martin Dominkus Rainer Kotz Theresia Thalhammer Klemens Trieb Teresa Zettl Christian F. Singer Received: 21 November 2009 /Accepted: 28 February 2010 /Published online: 26 March 2010 # Springer-Verlag 2010 Abstract Osteosarcoma is the most common primary chemotherapy resistance, angiogenesis, steroid- and neuro- malignant bone tumour. Currently osteosarcoma classifica- peptide hormone receptor activity, acute-phase response tion is based on histological appearance. It was the aim of and serotonin receptor activity and members of the Wnt/ß- this study to use a more systematic approach to osteosar- catenin pathway and many others. Furthermore, we coma classification based on gene expression analysis and validated the highly differential expression of six genes to identify subtype specific differentially expressed genes. including angiopoietin 1, IGFBP3, ferredoxin 1, BMP, We analysed the global gene expression profiles of ten decorin, and fibulin 1 in osteoblastic osteosarcoma relative osteosarcoma samples using Affymetrix U133A arrays (five to non-osteoblastic osteosarcoma. Our results show the osteoblastic and five non-osteoblastic osteosarcoma utility of gene expression analysis to study osteosarcoma patients). Differential gene expression analysis yielded 75 subtypes, and we identified several genes that may play a genes up-regulated and 97 genes