《IRE1α knockdown rescues tunicamycin-induced developmental defects and apoptosis in Xenopus laevis》.pdf

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《IRE1α knockdown rescues tunicamycin-induced developmental defects and apoptosis in Xenopus laevis》.pdf

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AvailableonlineatWWW．jbr-pub．org OpenAccessatPubMedCentral JBR TheJournalofBiomedicalResearch，2014，28(4)：275—28l ResearchPaper IREI~knockdownrescuestunicamycin--induceddevelopmental defectsandapoptosisinXenopuslaevis LiYuan，JinYu，XinxinLi，JiaojiaoFenga，ChenyangYin，XinruWang。，圜。DepartmentofBiochemistryandMolecularBiology,NanjingMedicalUniversity,Nanjing,Jiangsu210029,China； DepartmentofHematology,Friendshiphospitalofyilikazakhprefecture，Yining,Xinjiang83500~China；。KeyLaboratoryofReproductiveMedicine，InstituteofToxicology,NanjingMedicalUniversity,Nanjing,~angsu210029,China． Received06July2012，Revised06September2012，Accepted04December2013，Epub 15Mrach2014 Abstract Inositolrequiringenzyme一1fIRE1)ishighlyconservedfrom yeaststohumans．Uponendoplasmicreticulum fER)srtess．IRE1activatesX—box—bindingprotein1(XBP1)byunconventionalsplicingofXBP1mRNA，which activatesunfoldedproteinresponse(UPR)torestoreERhomeostasis．Inmice，IREleplaysanessentialrolein extraembryonictissues．However．itspreciseactionduringtheeralystageofdevelopmentisunknown．Inhtisstudy，山egainandloss—of-functionanalyseswereusedtoinvestigatethefunctionofXenopusIREI~t(xlREle)．The effectsofxlREI7duringembryodevelopmentweredetectedwithRT—PCR na dwholemountinsituhybridization． ERstresswasinducedbytunicamycin．TheapoptoticcellsweremeasuredbyTUNNELassays．Althoughbohtgain and1ossofxIREl。cfunctionhadnosignificna teffectonXenopusembryogenesis，knockdownofxlRElc~could rescuetunicamycin—induceddevelopmenta1defectsandapoptosis．ThefindingindicatesthatxlREI~isnotrequired forembryogenesisbutisrequiredofrtunicamycin—induceddevelopmentaldefectsandapoptosisinXenopuslaevis． Keywords：IREle，Xenopuslaevis，tunicamycin，developmentaldefects