《Postharvest Biological Control of Apple Anthracnose by Bacillus subtilis BS80-6,A Mutant Strain through Ion Implantation》.pdf

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2015-11-12 发布于河南
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《Postharvest Biological Control of Apple Anthracnose by Bacillus subtilis BS80-6,A Mutant Strain through Ion Implantation》.pdf

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PlantDiseasesandPests 2014，5(3)：15—19 PostharvestBiologicalControlofAppleAnthracnose byBacillussubtilisBS80-6，A MutantStrainthrough Ion Implantation TanGenjia ，FeiDan，ShiYangwei，LiuShufang，LiLi，LiZengzhi CoHegeofPlantProtection，AnhuiAgriculturalUniversity，Hefei230036，China Abstract rheoriginalBacillussubtiliswasisolatedfrom thesoilofanappleorchard．andB．subtilisstrainBs80-6．selectde from Bacillus subtilisbyionimplanta． tion，wasusedasanantagonistforpostharvestbiologicalcontrolagainst印plenathracnose(coUetotrichumgloeosporioides)．rIhemechanismsofactionnadefficacy ofBacillus subtilisstrainB$80-6 againstapplenathracnosecausde bycolletotrichumgloeosporioideswerestudide vitronadonapplesincontrollednadsemi—COB- mereialconditions．An印plicationofcellsuspension(10。cellspermE)ofthenatagonistinartificialwoundsof印piesreducde growthofc gloeosporioidesafter storageatdifferenttemperatures．1’heinhibitoryactionsofcellculturetohyphalgrowthna dtosporesgerminationwere86．6％ and98．65％，respectively，andthe controlefficacyofcellculture againstthediseasewas60．34％ atroom temperature．Theresultsshowde thatBs80-6 was moreeffcetiveagainstappleanthracnose． Th ecellcultureofBS80-6 receivedbettercontrolefficacyagainstaDpienathracnosethna culturefiltratesna dautoclavedcel1．A11treatmentssignificna dyinhibited thediseasebothinvitronadin 如ocomparedwithcontro1．Fruitstreatedwithcellculturena dstoredat10℃ hadlowerinfcetionrate．moredelayedformationof ace 1usnadsmallerlesiondiametert}lallhtosestorde at20oC．Therewas bettercontrolefficacyinfruitsinoculatedwithsporesafterapplicationofBS80-6．The mcehna ismsofBs80-6 activityappearedtoinvolveproductionofallantifungal substance．defomr ationofhyphal eellanddisintegrationofcel1wal1．Besides．BS80． 6couldimprovetheactivityofmajordefenseenzymesofapple，suchasperoxidase(POD)nadpolyphenoloxidase(PPO)． Keywords Colletotrichum gloeosporioides；Bac illus subtilisBS80-6 ：Bioeontml：Mechnaismsofaction Applepostharvestanthracnose(bitterrot)causedbycolleto-