氧化应激对断奶仔猪组织抗氧化酶活性与病理学变化的影响.pdfVIP

74 2009 1 29 1 Chin J Vet Sci Jan. 2009 Vol. 29 No . 1 1, 2 1* 袁施彬 , 陈代文 ( 1. , 625014; 2. , 637002) : 采用单因子试验设计, 探讨用 5% 氧化鱼油( 过氧化值为786. 50 meqO / kg) 和腹腔注射 12 mg/ kg 敌快 2 ( diq at) 对断奶仔猪肝脏抗氧化酶活性和组织病理学变化的影响试验共分3 个处理, 每 个处理8 个重复, 每 个重 复1头猪结果发现, 与新鲜鱼油组相比, 氧化鱼油和diq at 处理降低了试验猪肝脏抗氧化酶活性和提高了 MDA 含量, 降低了空肠绒毛高度和增加了隐窝深度, 空肠黏膜和肝脏发生不同程度的病理变化结果表明, 氧化鱼油和 diq at 都可以对断奶仔猪造成不同程度的氧化应激, 相对于氧化鱼油的氧化应激程度而言, 由diq at 诱导的氧化应 激程度更强一些 : 断奶仔猪; 氧化应激; 抗氧化酶; 病理变化 : S8522; S8523 : A : 1005-4545(2009) 01-0074-05 Effect of oxidativestress on theantioxidant enzymes andpathological changesin piglets YUAN Shi-bin1, 2 , CHEN Dai-w en1* ( 1. I nst it ute of A nimal N utr it ion, S ichuan A g r ic ultur al Uni- v ersity , Yaan, S ichuan 625014, China; 2. College of B iolog ical S ciences , China West N ormal Uni- v ersity , N anchong, Sichuan 637002, China Abstract: The experiment w as cond cted to st dy the effect of oxidat ive stress on the antioxidant enzymes and pathological changes in piglets.T wenty fo r w eanling pigs w ere allotted t o t hree gro ps of 8 each. According to a single factorial experimental design, the pigs received diets w ith 5% of either a fresh ( gro p 1 and gro p 3) or an ox- idized fish oil(peroxidative val e w as 786. 50 meqO / kg before incl sion in the diet) , at the begining of the experi- 2 ment, pigs in gro p 3 received an intraperitoneal injection of diq at at 12 mg/ kg of body w eight. The res lts showed that the activities of ant ioxidant enzymes in the liver decreased, and the content of MDA in the body tiss es in- creased, the villi height decreased and the crypt depth of the jej n m increased in gro p 2 and gro