水稻_9311_突变体筛选与突变体库构建.pdfVIP

32 10 Vol132, No110 2006 10 1525~ 1529 ACTA AGRONOMICA SINICA pp1 1525- 1529 Oct1, 2006 / 93110 * X 叶 俊 吴建国 杜 婧 郑 希 张 志 石春海 ( , 310029) : CEMS / 93110 , M M , 65 210 ( 2 3 675 ) , 5162% C EMS , , EMS : ; ; C; EMS( ) ; : S511 The Screening of Mutants and Construction of Mutant Population for Cultivar / 93110 in Rice (Oryza sativa L1) * YE Jun, WU Jian-Guo, DU Jing, ZHENG Xi, ZHANG Zhi and SHI Chun-Hai (Department of Agronomy, Zhejiang University, Hangzhou 310029, Zhejiang, China) Abstract: Rice has become an established model system in the last decade and the organism chosen in the study of the cereal genomes1 In order to find more mutants in the same genetic background, the seeds of indica rice cultivar 9311were treated with C-rays or EMS ( ethyl methan sulfonate) 1 Some mutants with the variations of leaf, stalk, panicle or root traits were found in the progenies, which themutation frequency was 5162%1 Four hundred and sixty-five out of the 675 mutants were induced by C-rays, and the other 210 mutants by EMS1 Comparing with the EMS mutagenesis population, more mutants were produced in C-rays mutagenesis population1 But some mutants, such as the purple leaf sheath mutant and lesion resembling disease mutant, were only found in EMS mutagenesis population1 The mutant library created will be helpful for gene identification and gene functional analysis1 eywords: Rice ( Oryza sativa L1) ; Mutagenesis; C-rays; EMS ( Ethyl Methan Sulfonate) ; Mutant , , [ 6-7] [ 7-9] ( 30 Mb, 1P5 1P