《二化性家蚕滞育发动期间滞育性卵和非滞育性卵的烟酰胺腺嘌呤二核苷酸变化》.pdfVIP

5A,tC●e仃ee 2013，39(1)：0047—0051 ISSN 0257—4799：CN32—1115／S E—mail：CYKE@chin~ourna1．net．cn 二化性家蚕滞育发动期 间滞育性卵和非滞 育性卵的烟酰胺腺 嘌呤二核苷酸变化 王启龙 姚金美 司马杨虎 赵林J (苏州大学基础医学与生物科学学院，江苏苏州 215123) 摘 要 为了探究家蚕滞育发动期间呼吸耗氧量下降与烟酰胺腺嘌呤二核苷酸 (NAD)含量变化的关系，用高效液相色谱法 和分光光度法分别检测在 25℃条件下产下后 12～72h的二化性家蚕滞育性卵与非滞育性卵中的还原型NAD(NADH)含量、 氧化型NAD(NAD )含量、乳酸脱氢酶 (LDH)活性和胞质苹果酸脱氢酶(cMDH)活性。滞育性卵中的NADH+NAD 含量、 NADH／NAD 值及 LDH活性分别在产下后 18、36、24h达到峰值 ，而 cMDH活性处于波动中。在产下后 24—72h，非滞育性卵 中的NADH／NAD 值极显著低于滞育性卵，而LDH和 cMDH活性极显著高于滞育性卵。据此可以推测，滞育发动期间家蚕滞 育性卵的NAD 降解增强和NAD 再生下降共同导致呼吸耗氧量下降。 关键词 家蚕；滞育发动；呼吸耗氧量；烟酰胺腺嘌呤二核苷酸；乳酸脱氢酶；胞质苹果酸脱氢酶 中图分类号 $881．2 文献标识码 A 文章编号 0257—4799(2013)01—0047—05 Change of Nicotinam ide Adenine Dinucleotide in Diapause and Non-diapauseEggsofBivoltineBombyxm oriDuringDiapauseIniti- ation WANG Qi—Long YAO Jin—Mei SIMAYang—Hu ZHAO Lin—Chuan (SchoolofBiologyandBasc『MedicineSciences，SoochowUniversity，SuzhouJiangsu215123，China) Abstract Inorderto investigatetherelationshipbetweenreductionOfrespiratoryoxygenconsumptionandchangeof nicotinamideadeninedinucleotide(NAD)contentduringdiapauseinitiationofthesilkworm (Bombyxmori)，reduced NAD (NADH)content，oxidizedNAD(NAD )content，lactatedehydrogenase(LDH)activityandcytosolicmalatede— hydrogenase(cMDH)activityindiapauseandnon—diapauseeggsofabivoltinesilkwormvarietyfrom 12hto72hafter ovipositionat25oc were determinedbyusinghigh performance liquid chromatographicand spectrOphOtOmetricmeth— ods．NADH +NAD content．NADH／NAD valueandLDH activity in diapauseeggsreachedthepeakvalueat18，36 and24hafterovipositionrespectively，whilecMDH activityremainedfluctuated．At24tO72haEeroviposition，NADH／ NAD valueinnon—diapause eggswasextremelysignificantly lowerthan thatin diapause eggs，while the contentsof LDH andcMDH innon—diapauseeggswasextremelysignificantlyhigherthanthatindiapauseeggs．Itisinferredthatthe enhancementofNAD degradationandthedeclineofNAD regenerationindiapauseeggsjointlyleadtothereduction ofres