Oxygen-glucose+deprivation+regulates+BACE1+expression+through+induction+of+autophagy+in+Neuro-2a-APP695+cells.pdf

NEURAL REGENERATION RESEARCH September 2015,Volume 10,Issue 9 RESEARCH ARTICLE Oxygen-glucose deprivation regulates BACE1 expression through induction of autophagy in Neuro-2a/APP695 cells 1 1 1 1 1 2 1 2 1, 2 1, * Rong-fu Chen , Ting Zhang , Yin-yi Sun , Ya-meng Sun , Wen-qi Chen , Nan Shi , Fang Shen , Yan Zhang , Kang-yong Liu , Xiao-jiang Sun 1 Department of Neurology, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China 2 Zhoupu Hospital, Pudong New District, Shanghai, China Abstract *Correspondence to: Our previous findings have demonstrated that autophagy regulation can alleviate the decline of Xiao-jiang Sun, Ph.D., learning and memory by eliminating deposition of extracellular beta-amyloid peptide (Aβ) in sunxj155@. the brain after stroke, but the exact mechanism is unclear. It is presumed that the regulation of beta-site APP-cleaving enzyme 1 (BACE1), the rate-limiting enzyme in metabolism of Aβ, would orcid: be a key site. Neuro-2a/amyloid precursor protein 695 (APP695) cell models of cerebral isch- 0000-0003-2061-7262 (Xiao-jiang Sun) emia were established by oxygen-glucose deprivation to investigate the effects of Rapamycin (an autophagy inducer) or 3-methyladenine (an autophagy inhibitor) on the expression of BACE1. doi: 10.4103/1673-5374.165511 Either oxygen-glucose deprivation or Rapamycin down-regulated the expression of