Free Zn2 + determination in systems with Zn-Glutathione》.pdfVIP

Journal of Electroanalytical Chemistry 756 (2015) 207 –211 Contents lists available at ScienceDirect Journal of Electroanalytical Chemistry journal homepage: /locate/jeac Free Zn2+ determination in systems with Zn-Glutathione Mireia Lao a, Angela Dago b, Nuria Serrano b, Encarna Companys a, Jaume Puy a, Josep Galceran a,⁎ a Departament de Química, Universitat de Lleida and AGROTECNIO, Rovira Roure 191, 25198 Lleida, Spain b Departament de Química Analítica, Universitat de Barcelona, Martí i Franqués 1, 08028 Barcelona, Spain a r t i c l e i n f o a b s t r a c t Article history: Zn-Glutathione speciation was studied applying the electrochemical technique AGNES (Absence of Gradients and Received 14 May 2015 Nernstian Equilibrium Stripping) to determine the free zinc concentration. In titrations varying either pH, total Received in revised form 14 August 2015 concentration of glutathione (cT,GSH ) or total concentration of Zn (cT,Zn ), free Zn concentrations determined Accepted 19 August 2015 with AGNES were compared with the values predicted from previously reported complexation constants. The Available online 28 August 2015 speciation of Zn was studied in a real sample of root extracts of Hordeum vulgare where the cT,Zn had been deter- Keywords: mined by ICP-MS and cT,GSH by HPLC. The free [Zn2+] was measured with AGNES using a special device where a Absence of Gradients and Nernstian mixture of N2/CO2 satura