Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses》.pdf

Industrial Crops and Products 74 (2015) 535–544 Contents lists available at ScienceDirect Industrial Crops and Products j o u rn al h omepage: w w w. elsev /locate/indcrop Production of butyric acid by Clostridium tyrobutyricum (ATCC25755) using sweet sorghum stalks and beet molasses Magnus Sjöblom a,∗ , Leonidas Matsakas a,b,1, Paul Christakopoulos a,b,2 , Ulrika Rova a,3 a Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, Sweden b Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Street, Zografou Campus, 15780 Athens, Greece a r t i c l e i n f o a b s t r a c t Article history: Enzymatically liquefied sweet sorghum stalks and beet molasses were evaluated for butyrate produc- Received 22 January 2015 tion using Clostridium tyrobutyricum in 1 L scale fed-batch fermentations. The hydrolysates used for the Received in revised form 27 April 2015 fermentations were prepared separately by liquefying the sorghum stalks at 50 ◦ C, pH 5.0 for 18 h, with Accepted 20 May 2015 ® 30% (w/v) DM content using the enzyme preparation Cellic CTec2 at an activity of 26.5 FPU/g DM. To Available online 12 June 2015 enhance sucrose consumption, the fermentations were supplemented with inver