Proteomic analysis of flooded soybean root exposed to aluminum oxide nanoparticles》.pdf

Journal of Proteomics 128 (2015) 280 –297 Contents lists available at ScienceDirect Journal of Proteomics journal h omepage: /locate/jprot Proteomic analysis of flooded soybean root exposed to aluminum oxide nanoparticles Ghazala Mustafa a,b, Katsumi Sakata c, Setsuko Komatsu a,b,⁎ a Graduate School of Life and Environmental Science, University of Tsukuba, Tsukuba 305-8572, Japan b National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan c Department of Life Science and Informatics, Maebashi Institute of Technology, Maebashi 371-0816, Japan a r t i c l e i n f o a b s t r a c t Article history: Aluminum oxide (Al O ) nanoparticles are used in agricultural products and cause various adverse growth ef- 2 3 Received 6 June 2015 fects on different plant species. To study the effects of Al2O3 nanoparticles on soybean under flooding stress, a Received in revised form 2 August 2015 gel-free proteomic technique was used. Morphological analysis revealed that treatment with 50 ppm Al2O3 nano- Accepted 15 August 2015 particles under flooding stress enhanced soybean growth compared to ZnO and Ag nanoparticles. A total of 172 Available online 22 August 2015 common proteins that significantly changed in abundance among control, flooding-stressed, and flooding- st