Proteomic analysis of immunogenic proteins from salivary glands of Aedes aegypti》.pdf

Journal of Infection and Public Health (2015) 8, 575—582 Proteomic analysis of immunogenic proteins from salivary glands of Aedes aegypti Rike Oktarianti a,b b,∗ c , Kartika Senjarini , Toshiya Hayano , Fatchiyah a d Fatchiyah , Aulanni’am a Biology Department, Faculty of Mathematics Natural Sciences, Brawijaya University, Indonesia b Biology Department, Faculty of Mathematics Natural Sciences, University of Jember, Indonesia c Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, Japan d Laboratory of Biochemistry, Chemistry Department, Faculty of Mathematics Natural Sciences, Brawijaya University, Indonesia Received 13 January 2015; received in revised form 1 April 2015; accepted 3 April 2015 KEYWORDS Summary Humans develop anti-salivary proteins after arthropod bites or exposure Proteomic analysis; to insect salivary proteins. This reaction indicates that vector bites have a positive Immunogenic proteins; effect on the host immune response, which can be used as epidemiological markers Salivary gland; of exposure to the vector. Our previous study identified two immunogenic proteins Aedes aegypti with molecular weights of 31 kDa and 56 kDa from salivary gland extract (SGE) of Aedes aegypti that cross-reacted with serum samples from Dengue Hemorrhagic Fever (DHF) patients and healthy people in an endemic area (Indonesia). Serum sam- ples from individuals living in non-endemic area (sub-tropical country) and infants did not show the immunogenic reactions. The objective