Proton pumping complex I increases growth yield in Candida utilis》.pdf

Biochimica et Biophysica Acta 1847 (2015) 1320–1326 Contents lists available at ScienceDirect Biochimica et Biophysica Acta j ournal homepa ge: /l ocate/bbabio Proton pumping complex I increases growth yield in Candida utilis Nicole Avéret, Marie-Lise Jobin, Anne Devin, Michel Rigoulet ⁎ Institute of Biochemistry and Genetics of the Cell, CNRS UMR 5095, 1 Rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France Université de Bordeaux, 1 Rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France a r t i c l e i n f o a b s t r a c t Article history: In living cells, growth is the result of coupling between substrate catabolism and multiple metabolic processes Received 26 March 2015 that take place during net biomass formation and cellular maintenance processes. A crucial parameter for growth Received in revised form 30 June 2015 evaluation is its yield, i.e. the efficiency of the transformation processes. The yeast Candida utilis is of peculiar Accepted 6 July 2015 interest since its mitochondria exhibit a complex I that is proposed to pump protons but also an external Available online 9 July 2015 NADH dehydrogenase that do not pump protons. Here, we show that in C. utilis cells grown on non-fermentable Keywords: media, growth yield is 30% higher as compared to that of Saccharomyces cerevisiae that do not exhibit a complex I. Yeast Moreover, ADP/O determination in C. utilis shows that electrons