Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves》.pdf
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Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves》.pdf
Food Chemistry 136 (2013) 1453–1460
Contents lists available at SciVerse ScienceDirect
Food Chemistry
journal homepage: www.elsevier .com/locate/foodchem
Purification, physicochemical characterisation and anticancer activity of a
polysaccharide from Cyclocarya paliurus leaves
Jian-Hua Xie a, Xin Liu a, Ming-Yue Shen a,b, Shao-Ping Nie a, Hui Zhang a, Chang Li a, De-Ming Gong a,c,
Ming-Yong Xie a,⇑
a State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
b Center of Analysis and Testing, Nanchang University, Nanchang 330047, China
c School of Biological Sciences, University of Auckland, Private Bag 92019, New Zealand
a r t i c l e i n f o a b s t r a c t
Article history: A Cyclocarya paliurus (Batal.) Iljinskaja polysaccharide (CPP) was isolated and purified by hot water
Received 7 April 2012 extraction, ethanol precipitation, deproteinisation and anion-exchange chromatography. Its physico-
Received in revised form 7 August 2012 chemical properties were characterised by gel permeation chromatography (GPC), gas chromatogra-
Accepted 12 September 2012
phy–mass spectrometry (GC–MS), thermal gravimetric analysis (TGA), Fourier transform infrared
Available online 29 September 2012
spectrometry (FTIR), UV–visible spectrophotometry, dynamic light scattering (DLS) and viscometry
analysis. The anticancer effect of CPP i
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